Paenibacillus terreus sp nov., isolated from forest soil

A Gram-stain-positive, rod-shaped, endospore-forming, motile bacterium, designated D33(T), was isolated from a forest soil sample. The strain grew optimally at 30-37 degrees C, pH 8.0 and with 1 % (w/v) NaCI. The 16S rRNA gene sequence of the isolate showed similarities lower than 97 % with respect to species of the genus Paenibacillus. Strain D33(T) contained meso-diaminopimelic acid in the cell-wall peptidoglycan, and ribose and lower amounts of glucose and galactose as the whole-cell sugars. The major cellular fatty acid was anteiso-C-15 (:) (0), and menaquinone-7 (MK-7) was the only respiratory quinone. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, two glycolipids and an unknown lipid. The DNA G + C content was 51.1 mol%. The low DNA DNA relatedness values between strain D33(T) and recognized species of the genus Paenibacillus, together with many phenotypic properties supported the classification of strain D33(T) as representative of a novel species of the genus Paenibacillus, for which the name Paenibacillus terreus sp. nov. is proposed. The type strain is D33(T) (=KACC 18491(T)=DSM 100035(T)=CCTCC AB 2015273(T)).