Brevundimonas albigilva sp nov., isolated from forest soil

Strain NHI-13(T), a Gram-stain-negative, aerobic and short rod-shaped bacterium, was isolated from forest soil at Kyonggi University in Suwon, South Korea. It grew optimally in R2A medium, at 20-30 degrees C, in the presence of 0-4 % NaCl. Colonies resulting from incubation of the strain on agar plates for 2 days were circular, raised, translucent, viscous and whitish-yellow, with entire margins. This strain exhibited high catalase activity but was negative for oxidase. 16S rRNA gene sequence analysis showed that strain NHI-13(T) formed a coherent cluster with members of the genus Brevundimonas. Its similarities were 98.0 % with Brevundimonas aurantiaca DSM 4731(T), 97.9 % with Brevundimonas vesicularis LMG 2350(T), 97.6 % with Brevundimonas intermedia ATCC 15262(T), 97.5 % with Brevundimonas nasdae GTC 1043(T), 97.1 % with 'Brevundimonas olei ' MJ15, 97.1 % with Brevundimonas mediterranea V4.BO.10(T) and 97.0 % with Brevundimonas poindexterae FWC40(T). The major cellular fatty acids were summed feature 8 (C-18 : 1 omega 7c/C-18 : 1 omega 6c), C-16 : 0 and 11-methyl C-18 : 1 omega 7c. The DNA G+C content was 63 mol%. The predominant quinone was ubiquinone Q-10. The polar lipid profile contained 1,2-di-O-acyl-3-O-alpha-D-glycopyranuronosyl glycerol, 1,2-di-O-acyl-3-O-alpha-D-glycopyranosyl glycerol, 1,2-di-O-acyl-3-O-[D-glycopyranosyl (1 -> 4)-alpha-D-glucopyranuronosyl] glycerols, phosphatidylglycerol, 1,2-diacyl-3-O-(6'-phosphatidyl-alpha-D-glucopyranosyl) glycerol and other unknown lipids. The DNA relatedness of strain NHI-13(T) with its reference strains was in the range of 43-56 %. On the basis of its phenotypic, genotypic, chemotaxonomic and phylogenetic distinctiveness, strain NHI-13(T) is suggested to be a representative of a novel species, belonging to the genus Brevundimonas. Therefore, the name Brevundimonas albigilva. sp. nov. is proposed, with the type strain being NHI-13(T) (=KEME 9005-016(T)=KACC 18249(T)=JCM 30385(T)).