Luteimonas tolerans sp nov., isolated from hexachlorocyclohexane-contaminated soil

A Gram-stain-negative, aerobic, rod-shaped, non-spore-forming, yellow pigmented bacterial strain (UM1(T)) was isolated from the hexachlorocyclohexane (HCH)-contaminated dumpsite located at Ummari village in Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UM1(T) belongs to the genus Luteimonas with Luteimonas aestuarii B9(T) as the closest neighbour (97.2 % 16S rRNA gene sequence similarity). The DNA G+ C content of strain UM1(T) was 64.3 mol%. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). Main fatty acids were iso-C-15 : 0, iso-C-11 : 0, iso-C-11 : 0 3-OH, iso-C-17 : 0 and summed feature 9 (C-16 : 0 10-methyl and/or iso-C-17 : 1 omega 9c). Ubiquinone (Q-8) was the only respiratory quinone. Spermidine was detected as the major polyamine. The DNA-DNA relatedness value of strain UM1(T) with respect to its closest neighbour Luteimonas aestuarii B9(T) was well below 70 % (similar to 49 %). Thus, data obtained from phylogenetic analysis, DNA-DNA hybridization, and chemotaxonomical and biochemical analyses supports classification of strain UM1(T) as representative of a novel species of the genus Luteimonas, for which the name Luteimonas tolerans sp. nov. is proposed. The type strain is UM1(T) (=DSM 28473(T) =MCC 2572(T) =KCTC 42936(T)).