期刊
IBRO NEUROSCIENCE REPORTS
卷 11, 期 -, 页码 144-155出版社
ELSEVIER
DOI: 10.1016/j.ibneur.2021.09.005
关键词
Muscarinic-1 receptor; Prelimbic; Prefrontal cortex; Adolescence; Marijuana; Cannabinoid
资金
- National Institute of Drug Abuse (NIDA) [DA042943, DA043982]
- Spanish Ministerio de Ciencia, Innovacion y Universidades [PRX18/00436]
- NIDA Drug Supply Program (Bethesda, MD, USA) [9-THC]
Long-term cannabis use during adolescence can have harmful effects on the brain, mainly due to the impact of marijuana components on the brain. This study found that the action of Delta 9-THC in the PL-PFC can affect the distribution and function of M1R, leading to cortical dysfunction.
Long-term cannabis use during adolescence has deleterious effects in brain that are largely ascribed to the activation of cannabinoid-1 receptors (CB1Rs) by delta-9-tetrahydrocannabinol (Delta 9-THC), the primary psychoactive compound in marijuana. Systemic administration of Delta 9-THC inhibits acetylcholine release in the prelimbic-prefrontal cortex (PL-PFC). In turn, PL-PFC acetylcholine plays a role in executive activities regulated by CB1R-targeting endocannabinoids, which are generated by cholinergic stimulation of muscarinic-1 receptors (M1Rs). However, the long-term effects of chronic administration of increasing doses of Delta 9-THC in adolescent males on the distribution and function of Ml and/or CB1 receptors in the PL-PFC remains unresolved. We used C57BL\6J male mice pre-treated with vehicle or escalating daily doses of Delta 9-THC to begin filling this gap. Electron microscopic immunolabeling showed M1R-immunogold particles on plasma membranes and in association with cytoplasmic membranes in varying sized dendrites and dendritic spines. These dendritic profiles received synaptic inputs from unlabeled, CB1R- and/or M1R-labeled axon terminals in the PL-PFC of both treatment groups. However, there was a size-dependent decrease in total (plasmalemmal and cytoplasmic) M1R gold particles in small dendrites within the PL-PFC of mice receiving Delta 9-THC. Whole cell current-clamp recording in PL-PFC slice preparations further revealed that adolescent pretreatment with Delta 9-THC attenuates the hyperpolarization and increases the firing rate produced by local muscarinic stimulation. Repeated administration of Delta 9-THC during adolescence also reduced spontaneous alternations in a Y-maze paradigm designed for measures of PFC-dependent memory function in adult mice. Our results provide new information implicating M1Rs in cortical dysfunctions resulting from adolescent abuse of marijuana.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据