期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 48, 期 5, 页码 2087-2097出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2016.3442
关键词
ovarian cancer; cancer-associated fibroblasts; cisplatin resistance; STAT3; p-Akt; cisplatin-induced chemokine (C-C motif) ligand 5
类别
资金
- National Basic Research Program of China (973 Program) [2015CB553903]
- Chinese 863 Program [2012AA02A507]
- National Nature and Science Foundation of China [81230038, 81272859, 81025011, 81090414, 81000979, 81101962]
Currently, acquired resistance to cisplatin (DDP) is a substantial obstacle to reducing the morbidity and mortality due to ovarian malignant tumors. Nevertheless, cisplatin plays a vital role in killing the tumor cells while it may also be a 'primer' involved in chemotherapy resistance. We found that the cisplatin-induced chemokine (C-C motif) ligand 5 (CCL5) secretion derived from cancer-associated fibroblasts (CAFs) promoted ovarian cancer cell resistance to cisplatin. Via a cytokine chip assay, we identified a spectrum of secreted proteins that were derived from the CAFs through cisplatin-induced treatment. Among these, CCL5 significantly attenuated the cytotoxic effect of cisplatin chemotherapy in vitro and in vivo. Additionally, CCL5 expression was also detected in 62 serous ovarian cancer patient tissue specimens using IHC, and the results demonstrated that chemotherapy resistant patients displayed higher expression of CCL5 than the chemo-sensitive patients (P<0.05). Mechanistically, we found that CCL5 notably increased STAT3 and Akt phosphorylation levels in ovarian cancer cells. These results indicated that cisplatin-induced CCL5 secretion derived from the CAFs may promote cisplatin resistance, which was mediated by regulation of the STAT3 and PI3K/Akt signal pathways.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据