期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 48, 期 6, 页码 2472-2478出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2016.3451
关键词
Y-box binding protein 1; schizophyllan; antisense; drug delivery system; lung cancer
类别
Silencing Y-box binding protein 1 (YB-1) can be an excellent target for cancer therapy and many lung cancer cells express the polysaccharide-recognition receptor Dectin-1. We designed a Dectin-1 targeting vehicle delivering YB-1-antisense DNA. First, we selected five optimal antisense DNA sequences to silence YB-1 from among 153 candidates. We chose the sequence closest to the start codon (AS014), and attached dA(40) to the 3' end; dA(40) promotes complex formation with a (3-(1 -> 3)-D-glucan called schizophyllan (SPG). The resultant complexes were applied to 12 human-oriented lung cancer cell lines, and cell viability was examined. The cell lines exhibited decreased viability and showed strong affinity to bind SPG, suggesting the AS014/SPG complex entered the cells via the Dectin-1 mediated pathway.
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