期刊
APL BIOENGINEERING
卷 5, 期 4, 页码 -出版社
AIP Publishing
DOI: 10.1063/5.0068126
关键词
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资金
- Howard Hughes Medical Institute
- Gordon and Betty Moore Foundation
- NIH at UNC-CH [NIBIB P41-EB002025]
- National Science Foundation (NSF) GRFP [DGE-1650116]
- Caroline H. and Thomas Royster Fellowship
- [P41-EB002025]
The cell nucleus is not just a location for DNA, but also a complex material that resists physical deformations and dynamically responds to external mechanical cues. The molecules responsible for nuclear mechanical properties can contribute to laminopathies and potentially play a role in cellular mechanotransduction and physical processes in cancer. Studying nuclear mechanics and the downstream biochemical consequences or their modulation requires a suite of complex assays for applying, measuring, and visualizing mechanical forces across diverse length, time, and force scales.
It is increasingly appreciated that the cell nucleus is not only a home for DNA but also a complex material that resists physical deformations and dynamically responds to external mechanical cues. The molecules that confer mechanical properties to nuclei certainly contribute to laminopathies and possibly contribute to cellular mechanotransduction and physical processes in cancer such as metastasis. Studying nuclear mechanics and the downstream biochemical consequences or their modulation requires a suite of complex assays for applying, measuring, and visualizing mechanical forces across diverse length, time, and force scales. Here, we review the current methods in nuclear mechanics and mechanobiology, placing specific emphasis on each of their unique advantages and limitations. Furthermore, we explore important considerations in selecting a new methodology as are demonstrated by recent examples from the literature. We conclude by providing an outlook on the development of new methods and the judicious use of the current techniques for continued exploration into the role of nuclear mechanobiology. (c) 2021 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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