期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 11, 期 -, 页码 269-283出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S95863
关键词
C-60; BADSCs; molecular mechanisms; myocardium
资金
- National Natural Science Funds for Distinguished Young Scholar [31125013]
- National Key Basic Research and Development Program of China [2011CB606206]
- International Cooperation and Exchanges NSFC [31320103914]
- Key Program of National Natural Science Foundation of China [31030032]
- National Natural Science Foundation of China [31370975, 31200733, 31100674, 31370987, 31470940]
- National High Technology Research and Development Program of China [SS2015AA020304]
- NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM [R21AA020304] Funding Source: NIH RePORTER
Zero-dimensional fullerenes can modulate the biological behavior of a variety of cell lines. However, the effects and molecular mechanisms of proliferation and cardiomyogenic differentiation in brown adipose-derived stem cells (BADSCs) are still unclear. In this study, we report the initial biological effects of fullerene-C-60 on BADSCs at different concentrations. Results suggest that fullerene-C-60 has no cytotoxic effects on BADSCs even at a concentration of 100 mu g/mL. Fullerene-C-60 improves the MAPK expression level and stem cell survival, proliferation, and cardiomyogenesis. Further, we found that the fullerene-C-60 modulates cardiomyogenic differentiation. Fullerene-C-60 improves the expression of cardiomyocyte-specific proteins (cTnT and a-sarcomeric actinin). At elevated concentration, fullerene-C-60 reduces the incidence of diminished spontaneous cardiac differentiation of BADSCs with time. At the genetic level, fullerene-C-60 (5 mu g/mL) also improves the expression of cTnT. In addition, fullerene-C-60 promotes the formation of gap junction among cells. These findings have important implications for clinical application of fullerenes in the treatment of myocardial infarction.
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