期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 38, 期 2, 页码 417-422出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2016.2640
关键词
cadmium; nuclear factor-B signaling; renal glomerular endothelial cells; c-Jun N-terminal kinase signaling; apoptosis
资金
- Science and Technology Development Plan of Shandong Province [2013GSF11805]
- National Natural Science Foundation of China [81370269]
- Shandong Taishan Scholarship
The kidney is one of the primary organs targeted by cadmium (Cd), a widely distributed environmental pollutant. The glomerular endothelium is the major component of the glomerular filtration barrier. However, the effects of Cd on glomerular endothelial cells remain largely unknown. For this purpose, we aimed to determine the effects of low dose Cd on the survival of human renal glomerular endothelial cells (HRGECs). Cultured HRGECs were exposed to 4 mu M cadmium chloride (CdCl2) and examined at different time-points. We found that Cd activates the nuclear factor-B (NF-B) pathway without inducing the apoptosis of HRGECs. Pre-treating the cells with pyrrolidine dithiocarbamate (PDTC), a potent NF-B inhibitor, prior to Cd exposure triggered extensive cell death (73.5%). In addition, Cd activates the c-Jun N-terminal kinase (JNK) pathway, and inhibition of the NF-B pathway significantly elevates Cd-induced JNK phosphorylation in HRGECs (p<0.01). The combination treatment of PDTC and SP600125, a JNK pathway inhibitor, increased the survival of Cd-stimulated HRGECs compared with those cells treated with PDTC alone (p<0.05). Taken together, these findings demonstrate that the NF-B pathway plays an essential role in maintaining the survival of Cd-exposed HRGECs.
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