OPTIMISATION OF BIOMASS, LIPID AND CARBOHYDRATE PRODUCTIVITIES IN Chlorella vulgaris FOR BIOFUEL PRODUCTION

Synthesis of lipid and carbohydrate in microalgae simultaneously is essential for cost-effective microalgae-based biofuel production. Statistical optimisation approaches of the Plackett-Burman design (PBD) and central composite design (CCD) were applied to optimise the biomass, lipid, and carbohydrate productivities of the C. vulgaris UPSI-JRM01. The results obtained from PBD shown that NO3- concentration, light intensity and NaHCO3 concentration were the significant factors that affecting biomass productivity. Through CCD, optimum biomass, lipid, and carbohydrate product . ties were obtained at 401.81 mg/L NO3-, 11238.20 lux light intensity, and 0.30 g/L NaHCO3, achieving the highest biomass productivity of 404.24 mg/L/day, highest lipid productivity of 65.3 mg/L/day, and highest carbohydrate productivity of 165.43 mg/L/day. The major fatty acid methyl esters (FAMEs) produced were palmitic acid (33.54%) and linoleic acid (30.29%), thus producing microalgae-based biodiesel with properties complying with international biodiesel standards.

Automated summary

This study applies statistical optimization methods to determine the effect of NO3- concentration, light intensity, and NaHCO3 concentration on the biomass, lipid, and carbohydrate productivities of C. vulgaris. The results provide optimal conditions for highest biomass, lipid, and carbohydrate productivities, enabling the production of microalgae-based biodiesel that complies with international standards.