Preparation of blood samples for electron microscopy: The standard protocol

Electron microscopy is a powerful tool to study biological samples at higher magnification. The higher magnifications achieved by the electron microscopes are helpful to the researchers to study surface morphology as well as cellular morphology of the samples. The blood sample surface morphology can be visualized at higher magnification by scanning electron microscope (SEM). For the examination of the blood cells at the cellular level, transmission electron microscopes (TEM) are used. In this article, we have described the step-by-step standard protocol for the preparation of blood samples for electron microscopy. The prepared blood samples can be visualized under SEM and TEM. The obtained electron micrographs of blood cells can be used for differential diagnosis of various diseases at the cellular level.

Automated summary

Electron microscopy is a powerful tool for studying biological samples at higher magnifications, with SEM used for surface morphology and TEM for cellular morphology. Blood samples can be prepared following a standard protocol for viewing under both SEM and TEM, with obtained electron micrographs aiding in differential diagnosis of various diseases at the cellular level.