期刊
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
卷 306, 期 8, 页码 697-706出版社
ELSEVIER GMBH
DOI: 10.1016/j.ijmm.2016.08.004
关键词
Lipopolysaccharide (LPS); Outer membrane vesicles (OMVs); Truncated LPS; Cross-protection; Mouse model
资金
- National Natural Science Foundation of China [31570928, 31472179, 31270981]
- National Institute of Allergy and Infectious Diseases of the National Institutes of Health [NIH R01 AI112680]
Salmonella enterica cause diarrheal and systemic diseases and are of considerable concern worldwide. Vaccines that are cross-protective against multiple serovars could provide effective control of Salmonella mediated diseases. Bacteria-derived outer membrane vesicles (OMVs) are highly immunogenic and are capable of eliciting protective immune responses. Alterations in lipopolysaccharide (LPS) length can result in outer membrane remodeling and composition of outer membrane proteins (OMPs) changing. In this study, we investigated the impact of truncated LPS on both the production and immunogenicity of Salmonella OMVs, including the ability of OMVs to elicit cross-protection against challenge by heterologous Salmonella strains. We found that mutations in waaJ and rjbP enhanced vesiculation, while mutations in waaC, waaF and waaG inhibited this process. Animal experiments indicated that OMVs from waaC, rfaH and rJbP mutants induced stronger serum immune responses compared to OMVs from the parent strain, while all elicited protective responses against the wild-type S. Typhimurium challenge. Furthermore, intranasal or intraperitoneal immunization with OMVs derived from the waaC and rfbP mutants elicited significantly higher cross-reactive IgG responses and provided enhanced cross protection against S. Choleraesuis and S. Enteritidis challenge than the wild-type OMVs. These results indicate that truncated-LPS OMVs are capable of conferring cross protection against multiple serotypes of Salmonella infection. (C) 2016 Elsevier GmbH. All rights reserved.
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