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Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies

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MICROBIOLOGY RESOURCE ANNOUNCEMENTS
卷 10, 期 46, 页码 -

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AMER SOC MICROBIOLOGY
DOI: 10.1128/MRA.00979-21

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  1. Japan Society for the Promotion of Science (JSPS) [19H00936, 19H05538]

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The complete genome of Thermus thermophilus strain TMY was resequenced using a hybrid assembly of long-read data from Oxford Nanopore Technologies and short-read data from MGI. Correction of errors in the previously reported genome sequence obtained solely from PacBio technology enabled high-quality comparative genomic analysis of closely related T. thermophilus genomes.
Complete genome resequencing was conducted for Thermus thermophilus strain TMY by hybrid assembly of Oxford Nanopore Technologies long-read and MGI short-read data. Errors in the previously reported genome sequence determined by PacBio technology alone were corrected, allowing for high-quality comparative genomic analysis of closely related T. thermophilus genomes.

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