期刊
ACS APPLIED NANO MATERIALS
卷 5, 期 2, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsanm.1c03783
关键词
Spin-coating; Nanostraw array; Oxide nanostraw; nano-electroporation; Intracellular delivery
资金
- National Key R&D Program of China [2021YFF1200700, 2021YFA0911100]
- National Natural Science Foundation of China [32171456, 32171399, 32171335, 61901535, 31900954, 62104264, 6210031309]
- Science and Technology Program of Guangzhou, China [201907010038, 202102080192, 202103000076]
- Guangdong Basic and Applied Basic Research Foundation [2019A1515012087, 2020A1515110940, 2021A1515012261, 2021A1515011609, 2020A1515111210]
- China Postdoctoral Science Foundation [2021M693686]
Nano-electroporation technology allows external biomolecules to enter cells using nanostraws. In this study, a simple spin-coating technique was developed to prepare oxide nanostraw arrays, which can be integrated with microfluidic devices for effective cell nano-electroporation. The fabricated nanostraws showed controllable diameters and spacing. They were successfully used for intracellular drug delivery, providing a unique solution for batch fabrication and serving as an essential module for biomedical studies.
Nano-electroporation technology allows external biomolecules to cross the cell membrane and enter cells. Nanostraws (NSs) with hollow channels are promising nanostructures to provide nano-electroporation for intracellular delivery, yet the preparation processes of NSs usually require intricated nanofabrication instruments and possess high requirements for operations. Here, a straightforward spin-coating processing technique was developed to prepare oxide NS arrays without relying on delicate instruments, which could be further integrated with microfluidic devices for effective cell nano-electroporation. The fabrication procedures of these NSs were simpler than the reported method relying on atomic layer deposition and Cl-2 reactive ion etching. By this spin-coating method, SiO2 and TiO2 NSs were successfully prepared with controllable NS diameters and spacing. SiO2 and TiO2 NSs were further integrated with a microfluidic system to build a nanoelectroporation system, which realized efficient intracellular drug delivery of cell membrane-impermeable molecules into both HeLa cells and MCF-7 cells. This strategy provides a unique solution for batch fabrication of NSs with a low-cost process, which could serve as the essential module of a nano-electroporation platform for various biomedical studies.
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