期刊
BIOSENSORS-BASEL
卷 12, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/bios12010030
关键词
bacteriophage (phage); biological detection; biosensor; nanomaterials; phage-based probe
资金
- National Natural Science Foundation of China [31870989, 137011016]
- Yangzhou University - Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD)
- Innovative and Entrepreneurial Team of Jiangsu Province
- Oklahoma Center for the Advancement of Science and Technology
This review provides an overview of the recent advances in phage-based probes for ultra-sensitive detection of various bio-species. It discusses the assembly of phages with metal nanoparticles to form an organic-inorganic hybrid probe and its unique plasmon resonance effect for visual detection. The review also highlights the editing technologies of phage genomes and discusses the potential scenarios for clinical application of phage-probe-based detection methods.
Bacteriophages, abbreviated as phages, have been developed as emerging nanoprobes for the detection of a wide variety of biological species, such as biomarker molecules and pathogens. Nanosized phages can display a certain length of exogenous peptides of arbitrary sequence or single-chain variable fragments (scFv) of antibodies that specifically bind to the targets of interest, such as animal cells, bacteria, viruses, and protein molecules. Metal nanoparticles generally have unique plasmon resonance effects. Metal nanoparticles such as gold, silver, and magnetism are widely used in the field of visual detection. A phage can be assembled with metal nanoparticles to form an organic-inorganic hybrid probe due to its nanometer-scale size and excellent modifiability. Due to the unique plasmon resonance effect of this composite probe, this technology can be used to visually detect objects of interest under a dark-field microscope. In summary, this review summarizes the recent advances in the development of phage-based probes for ultra-sensitive detection of various bio-species, outlining the advantages and limitations of detection technology of phage-based assays, and highlighting the commonly used editing technologies of phage genomes such as homologous recombination and clustered regularly interspaced palindromic repeats/CRISPR-associated proteins system (CRISPR-Cas). Finally, we discuss the possible scenarios for clinical application of phage-probe-based detection methods.
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