期刊
BIOSENSORS-BASEL
卷 12, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/bios12010021
关键词
cathepsin D; array SPRi; Ag; Au chip; blood serum; adhesive separating foil; glioblastoma
资金
- Polish Ministry of Education and Science [BST-162]
The study compared different material solutions for the determination of a specific biomarker in the blood serum of patients with glioblastoma and control samples, showing good linearity, LOQ, precision, and recovery rates. This effectively resolved the issue of accurately determining the levels of a specific biomarker in the serum of healthy individuals.
Non-fluidic array SPR imaging (SPRi) with appropriate biosensors is a new tool for the determination of various biomarkers in body fluids. Numerous biomarkers can be determined without signal enhancement or preliminarily preconcentration. The introduction of a new material solution of the chip may increase the scope of the application of this technique. Solutions with adhesive separating foil and an Ag/Au chip were compared with the previously used two-paint separating polymer and pure gold chip. These solutions were tested using the example of a biosensor for cathepsin D (Cath D), which consisted of pepstatin A (a Cath D inhibitor) immobilized via a cysteamine linker using the NHS/EDC protocol. Four material versions of the Cath D biosensor proved adequate in terms of range of linearity, LOQ, precision and recovery. All four versions of the biosensor were used for the determination of Cath D in the blood serum patients with glioblastoma and control samples, producing very similar results and showing an elevated biomarker concentration in the case of cancer. Therefore, the problem of determining the correct level of Cath D in the serum of healthy individuals has been resolved, correcting literature data which ranged over three orders of magnitude.
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