期刊
BIOSENSORS-BASEL
卷 12, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/bios12020071
关键词
dendrimers; gold nanoparticles; CpG-ODN; immunotherapy; T cells
资金
- Science and Technology Commission of Shanghai Municipality [19XD1400100, 21490711500, 20DZ2254900]
- National Natural Science Foundation of China [81761148028, 21875031]
- Shanghai Education Commission through the Shanghai Leading Talents Program
Cell-based immunotherapy, particularly T cell-mediated immunotherapy triggered by Au DENPs-MPC loaded with CpG-ODN, shows promising potential in completely eliminating cancer by enhancing the antigen presentation ability of BMDCs and activating T cells to inhibit tumor cell growth.
Recently, cell-based immunotherapy has become one of the most promising ways to completely eliminate cancer. The major challenge is to effectively promote a proper immune response to kill the cancer cells by activated T cells. This study investigated the effect of T cell-mediated immunotherapy trigged by Au DENPs-MPC (zwitterion 2-methacryloyloxyethyl phosphorylcholine (MPC)-functionalized dendrimer-entrapped gold nanoparticles) loading oli-godeoxynucleotides (ODN) of unmethylated cytosine guanine dinucleotide (CPG). Here, we first synthesized Au DENPs-MPC, evaluated their capability to compress and transfect CpG-ODN to bone marrow dendritic cells (BMDCs), and investigated the potential to use T cells stimulated by matured BMDCs to inhibit the growth of tumor cells. The developed Au DENPs-MPC could apparently reduce the toxicity of Au DENPs, and enhanced transfer CpG-ODN to the BMDCs for the maturation as demonstrated by the 44.41-48.53% increase in different surface maturation markers. The transwell experiments certificated that ex vivo activated T cells display excellent anti-tumor ability, which could effectively inhibit the growth of tumor cells. These results suggest that Au DENPs-MPC can deliver CpG-ODN efficiently to enhance the antigen presentation ability of BMDCs to activate T cells, indicating that T cells-based immunotherapy mediated by Au DENPs-MPC loaded with CpG-ODN may become the most promising treatment of cancer.
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