期刊
BIOSENSORS-BASEL
卷 12, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/bios12020121
关键词
SERS; Raman; microbeads; multiplex; immunoassays
资金
- Australian Research Council (ARC) [DP110102877]
A novel bioassay using Raman-coded antibody supports and surface-enhanced Raman scattering-coded nanotags was developed for accurate detection of multiple analytes from a single measurement, showing great potential for diverse biomedical applications.
Suspension microsphere immunoassays are rapidly gaining attention in multiplex bioassays. Accurate detection of multiple analytes from a single measurement is critical in modern bioanalysis, which always requires complex encoding systems. In this study, a novel bioassay with Raman-coded antibody supports (polymer microbeads with different Raman signatures) and surface-enhanced Raman scattering (SERS)-coded nanotags (organic thiols on a gold nanoparticle surface with different SERS signatures) was developed as a model fluorescent, label-free, bead-based multiplex immunoassay system. The developed homogeneous immunoassays included two surface-functionalized monodisperse Raman-coded microbeads of polystyrene and poly(4-tert-butylstyrene) as the immune solid supports, and two epitope modified nanotags (self-assembled 4-mercaptobenzoic acid or 3-mercaptopropionic acid on gold nanoparticles) as the SERS-coded reporters. Such multiplex Raman/SERS-based microsphere immunoassays could selectively identify specific paratope-epitope interactions from one mixture sample solution under a single laser illumination, and thus hold great promise in future suspension multiplex analysis for diverse biomedical applications.
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