期刊
FRONTIERS IN CARDIOVASCULAR MEDICINE
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcvm.2021.804111
关键词
percutaneous mitral valvuloplasty; MMP-1; collagen; inflammation; rheumatic heart disease
资金
- National Institutes of Health [R01 HL147095, R01 HL136431, R01 HL141917]
This study evaluated the histological and molecular characteristics of mitral valves in patients with rheumatic mitral stenosis who underwent emergency surgery after percutaneous mitral valvuloplasty (PMV) and compared them with patients who underwent elective mitral valve replacement surgery. The findings showed that inflammatory cells contribute to the accumulation of MMP-1 and IFN-gamma in valve leaflets, leading to uneven collagen remodeling and increased risk of leaflet tear.
Mitral regurgitation (MR) is a major complication of the percutaneous mitral valvuloplasty (PMV). Despite high technical expertise and cumulative experience with the procedure, the incidence rate of severe MR has not decreased. Although some of MR can be anticipated by echocardiographic analysis; leaflet tearing, which leads to the most dreaded type of MR, remains unpredictable. Irregular valvular collagen remodeling is likely to compromise tissue architecture and increase the tearing risk during PMV balloon inflation. In this study, we evaluated histological and molecular characteristics of excised mitral valves from patients with rheumatic mitral stenosis (MS) who underwent emergency surgery after PMV due to severe MR caused by leaflet tear. Those findings were compared with patients who underwent elective mitral valve replacement surgery owing to severe MS, in whom PMV was not indicated. In vitro assay using peripheral blood mononuclear cells was performed to better understand the impact of the cellular and molecular alterations identified in leaflet tear mitral valve specimens. Our analysis showed that focal infiltration of inflammatory cells contributes to accumulation of MMP-1 and IFN-gamma in valve leaflets. Moreover, we showed that IFN-gamma increase the expression of MMP-1 in CD14(+) cells (monocytes) in vitro. Thus, inflammatory cells contribute to unevenly remodel collagen resulting in variable thickening causing abnormalities in leaflet architecture making them more susceptible to laceration.
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