期刊
CELL DEATH DISCOVERY
卷 7, 期 1, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s41420-021-00640-8
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资金
- National Natural Science Foundation of China [81572337, 81672471]
- Outstanding Young and Middle-aged Talents Support Program of the First Affiliated Hospital of Nanjing Medical University
- Innovation Capability Development Project of Jiangsu Province [BM2015004]
- Jiangsu Key Medical Discipline (General Surgery) [ZDXKA2016005]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [JX10231801]
The study found that SOX2OT binds to FUS through its 5' and 3' regions, resulting in FUS ubiquitination and degradation, thus promoting migration, invasion, tumor growth, and metastasis of pancreatic cancer cells. A better understanding of the SOX2OT-FUS regulatory axis is important for clarifying the mechanism of action of SOX2OT and providing new ideas for pancreatic cancer treatment.
Pancreatic cancer is a highly aggressive and lethal digestive system malignancy. Our previous studies revealed the correlation of high levels of lncRNA SOX2OT expression with patients' poor survival outcomes, the promoting role of SOX2OT in proliferation and cycle progression of pancreatic cancer cells, and the in vivo binding of SOX2OT to RNA binding protein FUS, which destabilized the protein expression of FUS. However, the mechanism of SOX2OT binding and inhibiting FUS protein stability remains unclear. In this study, we performed RNA pull-down, cycloheximide-chase, and ubiquitination assays to determine the effect of SOX2OT on FUS ubiquitination, and explored the specific regulatory mechanism of SOX2OT-FUS axis in pancreatic cancer cell migration, invasion, in vivo tumor growth, and metastasis through RNA sequencing. We found that SOX2OT binds to FUS through its 5 ' and 3 ' regions, resulting in FUS ubiquitination and degradation. The SOX2OT-FUS regulatory axis promotes migration, invasion, tumor growth, and metastasis ability of pancreatic cancer cells. The in-depth elaboration of the SOX2OT-FUS regulatory axis in pancreatic cancer may clarify the mechanism of action of SOX2OT and provide new ideas for pancreatic cancer treatment.
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