期刊
PHARMACEUTICALS
卷 15, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/ph15010099
关键词
Morus nigra; microwave hydrodiffusion and gravity; polyphenols; sugar content; alpha-glucosidase; lipase; xanthine oxidase; DPPH radical scavenging; central composite design
This study optimized a new method for extracting polyphenols and sugars from black mulberry and compared it with traditional extraction methods. The results showed that the optimized method performed better in terms of polyphenol content and lipase inhibition, while the traditional method showed better antioxidant and blood glucose regulation activities.
Black mulberry, Morus nigra L. (family: Moraceae), is a healthy food and medicinal plant. Microwave hydrodiffusion and gravity (MHG) is one of the most innovative applications of solvent-free microwave extraction. The aim of this study was to optimize for the first time the MHG solvent-free extraction of polyphenols and sugars from M. nigra fruits. Optimization was carried out using a central composite design (CCD) with selected responses such as extraction yield, total polyphenol (TPC), flavonoid (TFC), anthocyanin (TAC), and sugar (TSC) contents, in addition to DPPH radical scavenging, and alpha-glucosidase (AGHi), lipase (Li), and xanthine oxidase (XOi) inhibition as tools to evaluate the best parameters for efficient and rapid extraction of black mulberry. The optimized extract was characterized in terms of the aforementioned parameters to validate the models, and was further analyzed for 36 individual polyphenols using HPLC-MS/MS. The optimized MHG extract was finally compared with traditional extracts, and demonstrated much better performance in terms of TPC, TAC, and Li, while the traditional extracts showed better XOi and AGHi. In conclusion, MHG is a valuable green technique for the production of non-degraded black mulberry polyphenol-rich extract and we suggest its larger use in the pharmaceutical and food industries.
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