期刊
ISCIENCE
卷 25, 期 3, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2022.103883
关键词
-
资金
- National Natural Science Foundation of China [82125001, 81872290, 82073154, 81972172, 81772763, 81772465]
- Shanghai Science and Technology Committee [19XD1423200, 18140903900]
This study reveals the adaptive characteristics of the tumor microenvironment (TME) in early lung adenocarcinoma, including high CD4(+) T cell infiltration, high B-cell activation, and low CD8(+) T cell infiltration. Multiplex immunohistochemistry staining confirms the formation of tertiary lymphoid structures (TLS) and the enrichment of follicular regulatory T cells (Tfr) in TLS follicles, which may contribute to the attenuated anti-tumor immunity in early lung adenocarcinoma.
Knowledge of the tumor microenvironment (TME) in patients with early lung cancer,especially in comparison with the matched adjacent tissues, remains lacking.To characterize TME of early-stage lung adenocarcinoma, we performed RNA-seq profiling on 58 pairs of minimally invasive adenocarcinoma (MIA) tumors and matched adjacent normal tissues. MIA tumors exhibited an adaptive TME characterized by high CD4(+) T cell infiltration, high B-cell activation, and low CD8(+) T cell infiltration. The high expression of markers for B cells, activated CD4(+) T cells, and follicular helper T (Tfh) cells in bulk MIA samples and three independent single-cell RNA-seq datasets implied tertiary lymphoid structures (TLS) formation. Multiplex immunohistochemistry staining validated TLS formation and revealed an enrichment of follicular regulatory T cells (Tfr) in TLS follicles,which may explain the lower CD8+ T cell infiltration and attenuated anti-tumor immunity in MIA. Our study demonstrates how integrating transcriptome and pathology characterize TME and elucidate potential mechanisms of tumor immune evasion.
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