SARS-CoV-2 integral membrane proteins shape the serological responses of patients with COVID-19

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has elicited a unique mobilization of the scientific community to develop efficient tools to understand and combat the infection. Like other coronavirae, SARS-CoV-2 hijacks host cell secretory machinery to produce viral proteins that compose the nascent virions; including spike (S), envelope (E), and membrane (M) proteins, the most exposed transmembrane proteins to the host immune system. As antibody response is part of the anti-viral immune arsenal, we investigate the immunogenic potential of S, E, and M using a human cell-based system to mimic membrane insertion and N-glycosylation. Both S and M elicit specific Ig production in patients with SARS-CoV-2. Patients with moderate and severe diseases exhibit elevated Ig responses. Finally, reduced Ig binding was observed with spike G614 compared to D614 variant. Altogether, our assay points toward an unexpected immune response against M and represents a powerful tool to test humoral responses against actively evolving SARS-CoV-2 variants and vaccine effectiveness.

Automated summary

The SARS-CoV-2 pandemic has prompted the scientific community to develop tools to combat the infection by studying the immunogenic potential of viral proteins S, E, and M. Patients with moderate to severe diseases exhibit elevated Ig responses against these proteins, with reduced Ig binding observed with the spike G614 variant compared to the D614 variant. This research represents a powerful tool to test humoral responses against evolving SARS-CoV-2 variants and vaccine effectiveness.