期刊
ISCIENCE
卷 24, 期 12, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2021.103438
关键词
-
资金
- Centro Interdipartimentale di Servizi per la Ricerca che utilizza Animali da Laboratorio''-C.I. R. S.A.L
- Centro PiattaformeTecnologiche''-CPT (University of Verona)
- European Union [824164]
- Fondazione Telethon-Italy [GGP19250, GSP20004_ PAsMCT8006]
- Italian patient association la Colonna and GALM
- University of Verona [DDSP-FUR-6616]
- University of Milan [BIOMETRA15-6-3003005-1, PSR2018_RIVA_BIFARI]
The researchers have successfully developed a highly standardized, reproducible, and fast murine brain organoid model starting from embryonic neural stem cells. The brain organoids differentiated progressively and self-organized into 3D networks of functional neurons with a dorsal forebrain phenotype during development.
Brain organoids are in vitro three-dimensional (3D) self-organized neural structures, which can enable disease modeling and drug screening. However, their use for standardized large-scale drug screening studies is limited by their high batch-to-batch variability, long differentiation time (10-20 weeks), and higg production costs. This is particularly relevant when brain organoids are obtained from human induced pluripotent stem cells (iPSCs). Here, we developed, for the first time, a highly standardized, reproducible, and fast (5 weeks) murine brain organoid model starting from embryonic neural stem cells. We obtained brain organoids, which progressively differentiated and self-organized into 3D networks of functional neurons with dorsal forebrain phenotype. Furthermore, by adding the morphogen WNT3a, we generated brain organoids with specific hippocampal region identity. Overall, our results showed the establishment of a fast, robust and reproducible murine 3D in vitro brain model that may represent a useful tool for high-throughput drug screening and disease modeling.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据