期刊
ISCIENCE
卷 25, 期 3, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2022.103936
关键词
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资金
- Japan Science and Technology Agency Precursory Research for Embryonic Science and Technology (JST PRESTO) [JPMJPR17G6]
- Japan Science and Technology Agency Core Research for Evolutional Science and Technology (JST CREST) [JPMJCR1872]
- Japan Society for the Promotion of Science [20K20593, 20H02881]
Using SRS microscopy, we directly visualize the interactions of a deuterated analog of propofol in living cells, supporting the theory that propofol primarily interacts with the plasma membrane of neurons through non-specific binding. Additionally, we demonstrate that SRS microscopy can be used to monitor the dynamics of propofol binding in real-time.
The consensus for the precise mechanism of action of general anesthetics is through allosteric interactions with GABA receptors in neurons. However, it has been speculated that these anesthetics may also interact with the plasma membrane on some level. Owing to the small size of anesthetics, direct visualization of these interactions is difficult to achieve. We demonstrate the ability to directly visualize a deuterated analog of propofol in living cells using stimulated Raman scattering (SRS) microscopy. Our findings support the theory that propofol is highly concentrated and interacts primarily through non-specific binding to the plasma membrane of neurons. Additionally, we show that SRS microscopy can be used to monitor the dynamics of propofol binding using real-time, live-cell imaging. The strategy used to visualize propofol can be applied to other small molecule drugs that have been previously invisible to traditional imaging techniques
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