期刊
JOURNAL OF FUNGI
卷 7, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/jof7100854
关键词
aquatic fungi; differential expression; lignocellulose; laccase; RNA-Seq
资金
- Helmholtz-Association of German Research Centres
- Leibniz Association Pakt/SAW project MycoLink [SAW-2014-IGB-1]
- German Federal Ministry of Education and Research (BMBF, Forderkennzeichen) [033W034A]
- Community Science Program of the Joint Genome Institute (JGI) [CSP 1663]
- Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]
Fungi play a significant role in the decomposition of lignocellulose in ecosystems. The study focused on a specifically aquatic ascomycete, Clavariopsis aquatica, and found that it has the ability to modify lignin and use the carbohydrate components of lignocellulose as carbon and energy sources. The research sheds light on the lignocellulose degradation capabilities of aquatic fungi.
Fungi are ecologically outstanding decomposers of lignocellulose. Fungal lignocellulose degradation is prominent in saprotrophic Ascomycota and Basidiomycota of the subkingdom Dikarya. Despite ascomycetes dominating the Dikarya inventory of aquatic environments, genome and transcriptome data relating to enzymes involved in lignocellulose decay remain limited to terrestrial representatives of these phyla. We sequenced the genome of an exclusively aquatic ascomycete (the aquatic hyphomycete Clavariopsis aquatica), documented the presence of genes for the modification of lignocellulose and its constituents, and compared differential gene expression between C. aquatica cultivated on lignocellulosic and sugar-rich substrates. We identified potential peroxidases, laccases, and cytochrome P450 monooxygenases, several of which were differentially expressed when experimentally grown on different substrates. Additionally, we found indications for the regulation of pathways for cellulose and hemicellulose degradation. Our results suggest that C. aquatica is able to modify lignin to some extent, detoxify aromatic lignin constituents, or both. Such characteristics would be expected to facilitate the use of carbohydrate components of lignocellulose as carbon and energy sources.
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