期刊
BIOMEDICINES
卷 9, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines9111727
关键词
Enpp2; dendritic cells; migration
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2016R1D1A1B04932158]
- National Research Foundation of Korea [2016R1D1A1B04932158] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study investigated the biological effects of Enpp2 on dendritic cells (DCs) and found that Enpp2 is essential for the migration capacity of mature DCs (mDCs) to lymph nodes, enhancing our understanding of DC biology. Regulating Enpp2 may improve DC migration to lymph nodes, thereby enhancing the effectiveness of cancer vaccines based on DCs.
Enpp2 is an enzyme that catalyzes the conversion of lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA), which exhibits a wide variety of biological functions. Here, we examined the biological effects of Enpp2 on dendritic cells (DCs), which are specialized antigen-presenting cells (APCs) characterized by their ability to migrate into secondary lymphoid organs and activate naive T-cells. DCs were generated from bone marrow progenitors obtained from C57BL/6 mice. Enpp2 levels in DCs were regulated using small interfering (si)RNA or recombinant Enpp2. Expression of Enpp2 in LPS-stimulated mature (m)DCs was high, however, knocking down Enpp2 inhibited mDC function. In addition, the migratory capacity of mDCs increased after treatment with rmEnpp2; this phenomenon was mediated via the RhoA-mediated signaling pathway. Enpp2-treated mDCs showed a markedly increased capacity to migrate to lymph nodes in vivo. These findings strongly suggest that Enpp2 is necessary for mDC migration capacity, thereby increasing our understanding of DC biology. We postulate that regulating Enpp2 improves DC migration to lymph nodes, thus improving the effectiveness of cancer vaccines based on DC.
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