期刊
BIOMEDICINES
卷 10, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines10030529
关键词
childhood obesity; biomarkers; LncRNAs; miRNAs; Real-Time PCR
资金
- Andrea Ripoli Dirigente Analista Fondazione Gabriele Monasterio Regione Toscana CNR
This study aimed to identify inflammatory LncRNAs in obese adolescents and evaluate their expression differences compared to normal-weight adolescents. The results showed that only SNHG12 had significantly lower expression in obese adolescents. Circulating miRNAs showed an increasing trend in obese subjects, except for miR-181a expression. LncRNAs and miRNAs were correlated with some clinical and metabolic parameters.
Background and Methods: Long non-coding RNAs (LncRNAs) and microRNAs are involved in the pathogenesis of obesity, a multifactorial disease that is characterized by inflammation, cardiometabolic complications, and increased cancer risk among other co-morbidities. The up/down regulation of LncRNAs and microRNAs may play an important role in this condition to identify new diagnostic/prognostic markers. The aim of the study was to identify circulating inflammatory LncRNAs in obese adolescents (n = 54) and to evaluate whether their expression behaved differently compared to normal-weight adolescents (n = 26). To have a more complete insight, the expression of some circulating miRNAs that are linked to obesity (miR-33a, miR-223, miR-142, miR-199a, miR-181a, and miR-4454) were also analyzed. Results: LncRNAs and miRNAs were extracted simultaneously from plasma samples and amplified by Real-Time PCR. Among the 86 LncRNAs that were analyzed with custom pre-designed plates, only four (RP11-347E10.1, RP11-10K16.1, LINC00657, and SNHG12) were amplified in both normal-weight and obese adolescents and only SNHG12 showed significantly lower expression compared to the normal-weight adolescents (p = 0.026). Circulating miRNAs showed a tendency to increase in obese subjects, except for miR-181a expression. LncRNAs and miRNAs correlated with some clinical and metabolic parameters. Conclusions: Our results suggest the importance of these new biomarkers to better understand the molecular mechanisms of childhood obesity and its metabolic disorder.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据