期刊
BIOMEDICINES
卷 10, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines10020409
关键词
gut microbiota; inflammation; oxidative stress
资金
- National Funds by the Portuguese Foundation for Science and Technology (FCT) [UIDB/04033/2020, UIDB/CVT/00772/2020]
- FCT [2020.04789.BD, SFRH/BD/136747/2018]
- [2020.04789]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/136747/2018, 2020.04789.BD] Funding Source: FCT
This study aimed to characterize an animal model of colorectal cancer (CRC) in the early stages of disease development. DMH treatment induced epithelial dysplasia and inflammatory infiltrate in the intestines. Additionally, DMH treatment altered the composition of the gut microbiota. The findings suggest the importance of this model in studying chemoprevention in early-stage CRC.
This study aimed to characterize an animal model of colorectal cancer (CRC) in the early stages of disease development. Twenty-nine male Wistar rats were divided into two control groups (CTRL1 and CTRL2), receiving EDTA-saline injections and two induced groups (CRC1 and CRC2), receiving 1,2-dimethylhydrazine (DMH) injections for seven consecutive weeks. CRC1 and CTRL1 were euthanized at the 11th week, while CRC2 and CTRL2 were euthanized at the 17th week. DMH treatment decreased microhematocrit values and IL-6, ghrelin, and myostatin serum levels. Histopathological analysis of intestinal sections showed that DMH-treated rats were characterized by moderate to severe epithelial dysplasia. An adenoma was observed in one animal (CRC2 group), and the presence of inflammatory infiltrate at the intestinal level was primarily observed in DMH-treated animals. DMH also induced Ki-67 immunoexpression. The gut microbiota analysis showed a higher abundance of Firmicutes, Clostridia, Clostridiales, Peptostreptococcaceae, Blautia, Romboutsia, and Clostridium sensu stricto in CRC than CTRL rats, whereas Prevotellaceae, Prevotella, Akkermansia, and Lactobacillus levels were more prevalent in CTRL animals. Our results suggest that this model could be helpful to investigate chemoprevention in the early stages of CRC.
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