4.7 Article

Initial Biological Assessment of Upconversion Nanohybrids

期刊

BIOMEDICINES
卷 9, 期 10, 页码 -

出版社

MDPI
DOI: 10.3390/biomedicines9101419

关键词

upconversion nanoparticles; cucurbituril; cytotoxicity

资金

  1. MINECO [PID2020-115710GB-I00]
  2. Agencia Estatal de Investigacion-AEI [CEX2019-000919-M]
  3. Generalitat Valenciana [PROMETEO/2018/138, IDIFEDER/2018/064, CIDEGENT/2018/01]
  4. FEDER funds
  5. Ministerio de Educacion, Cultura y Deporte
  6. Universitat de Valencia (Programa propi del Vicerectorat d'Investigacio) [UV-INV-AE-1547619]
  7. Universitat de Valencia (Subprograma Atraccio de Talent Contractes Postdoctorals)
  8. European Union [794410]
  9. COST Action [CM1403]
  10. Marie Curie Actions (MSCA) [794410] Funding Source: Marie Curie Actions (MSCA)

向作者/读者索取更多资源

Research demonstrates that upconversion nanoparticles coated with cucurbit[7]uril (CB[7]) show no cytotoxicity or endotoxin contamination in endothelial cells for biological applications. However, higher cytotoxicity is observed in other cell types, possibly due to differences in lysosome content and particle uptake rate.
Nanoparticles for medical use should be non-cytotoxic and free of bacterial contamination. Upconversion nanoparticles (UCNPs) coated with cucurbit[7]uril (CB[7]) made by combining UCNPs free of oleic acid, here termed bare UCNPs (UCn), and CB[7], i.e., UC@CB[7] nanohybrids, could be used as photoactive inorganic-organic hybrid scaffolds for biological applications. UCNPs, in general, are not considered to be highly toxic materials, but the release of fluorides and lanthanides upon their dissolution may cause cytotoxicity. To identify potential adverse effects of the nanoparticles, dehydrogenase activity of endothelial cells, exposed to various concentrations of the UCNPs, was determined. Data were verified by measuring lactate dehydrogenase release as the indicator of loss of plasma membrane integrity, which indicates necrotic cell death. This assay, in combination with calcein AM/Ethidium homodimer-1 staining, identified induction of apoptosis as main mode of cell death for both particles. The data showed that the UCNPs are not cytotoxic to endothelial cells, and the samples did not contain endotoxin contamination. Higher cytotoxicity, however, was seen in HeLa and RAW 264.7 cells. This may be explained by differences in lysosome content and particle uptake rate. Internalization of UCn and UC@CB[7] nanohybrids by cells was demonstrated by NIR laser scanning microscopy.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据