期刊
BIOMEDICINES
卷 9, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines9111592
关键词
mesenchymal stem cells; biodistribution; nonlinear magnetic response; superparamagnetic iron oxide nanoparticles; multiforme glioblastoma; C6 glioma; magnetic resonance imaging; targeted drug delivery
资金
- Ministry of Science and Higher Education of the Russian Federation [0103-2019-0012)]
- DFG [SFB824/3, STA1520/1-1]
- program Open Access Publishing
- Technische Universitaet Muenchen (TUM) within the DFG funding
The study demonstrates that MSCs have high migratory capacity towards C6 glioblastoma cells and exhibit tumor-tropism. SPION-labeled MSCs can accumulate in tumor tissues in vivo and enhance the contrast of the tumor through high-field magnetic resonance imaging. Biodistribution studies support the retention of MSCs in glioblastoma.
Despite multimodal approaches for the treatment of multiforme glioblastoma (GBM) advances in outcome have been very modest indicating the necessity of novel diagnostic and therapeutic strategies. Currently, mesenchymal stem cells (MSCs) represent a promising platform for cell-based cancer therapies because of their tumor-tropism, low immunogenicity, easy accessibility, isolation procedure, and culturing. In the present study, we assessed the tumor-tropism and biodistribution of the superparamagnetic iron oxide nanoparticle (SPION)-labeled MSCs in the orthotopic model of C6 glioblastoma in Wistar rats. As shown in in vitro studies employing confocal microscopy, high-content quantitative image cytometer, and xCelligence system MSCs exhibit a high migratory capacity towards C6 glioblastoma cells. Intravenous administration of SPION-labeled MSCs in vivo resulted in intratumoral accumulation of the tagged cells in the tumor tissues that in turn significantly enhanced the contrast of the tumor when high-field magnetic resonance imaging was performed. Subsequent biodistribution studies employing highly sensitive nonlinear magnetic response measurements (NLR-M-2) supported by histological analysis confirm the retention of MSCs in the glioblastoma. In conclusion, MSCs due to their tumor-tropism could be employed as a drug-delivery platform for future theranostic approaches.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据