4.6 Article

B-Cell Deficiency Exacerbates Inflammation and Bone Loss in Ligature-Induced Experimental Periodontitis in Mice

期刊

JOURNAL OF INFLAMMATION RESEARCH
卷 14, 期 -, 页码 5367-5380

出版社

DOVE MEDICAL PRESS LTD
DOI: 10.2147/JIR.S330875

关键词

periodontitis; experimental periodontitis; alveolar bone loss; B lymphocytes

资金

  1. National Natural Science Foundation of China [81470740]

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B-cell deficiency exacerbates inflammation and alveolar bone loss in ligature-induced experimental periodontitis in mice, suggesting a role for B cells in the initiation of periodontitis. This is associated with increased osteoclast activity, upregulated RANKL expression, and a shift in gingiva cytokine expression profiles to Th1 and Th17. Additionally, a reduced CD4(+)/CD8(+) T cell ratio was observed in the B cell-deficient mice.
Objective: Periodontitis, one of the most prevalent chronic oral infectious diseases in humans, is induced by the breakdown in the balance between the biofilm and host immune system. Previous studies have shown the presence of large numbers of B cells in periodontitis lesions, implicating that B lymphocytes play a predominant role during the pathogenesis of periodontitis. This study aimed to investigate the role of all B cells in the initiation of periodontitis. Methods: Experimental periodontitis was induced in B cell-deficient (CD19Cre) mice and wild-type (WT) control mice by 5-0 silk ligation around the maxillary second molar. Four weeks after ligation, alveolar bone loss was determined by micro-computed tomography. The levels of inflammatory cytokines and receptor activator of NF-kappa B ligand (RANKL)/osteo-protegerin in periodontal lesions were analyzed using real-time quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. Lymphocyte populations in the cervical lymph nodes and spleen and among the peripheral blood mononuclear cells were detected by flow cytometry. Results: B-cell deficiency resulted in increased severity of alveolar bone loss in mouse experimental periodontitis, which was associated with increased osteoclast activity and upregulated RANKL expression in the periodontal lesions. In addition, gingiva cytokine expression profiles were shifted to T helper type 1 (Th1) and Th17 in the CD19Cre mice with ligature-induced periodontitis compared with WT mice. In addition, a reduced CD4(+)/CD8(+) T cell ratio was observed in the CD19Cre mice. Conclusion: B-cell deficiency exacerbates the inflammation and alveolar bone loss in ligature-induced experimental periodontitis in mice, implicating that B cells may overall a role in the initiation of periodontitis.

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