期刊
BIOACTIVE MATERIALS
卷 6, 期 12, 页码 4531-4541出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2021.05.008
关键词
Peptide polymer; Host defense peptide; Antimicrobial surface; MRSA; Subcutaneous infection
资金
- National Natural Science Foundation of China [22075078, 21774031]
- National Key Research and Development Program of China [2016YFC1100401]
- Program of Shanghai Academic/Technology Research Leader [20XD1421400]
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University
- Natural Science Foundation of Jiangsu Province [BK20180093]
- Natural Science Foundation of Shanghai [18ZR1410300]
- Research program of State Key Laboratory of Bioreactor Engineering
- Fundamental Research Funds for the Central Universities [22221818014]
The peptide polymer-modified thermoplastic polyurethane (TPU) surfaces exhibit broad-spectrum antibacterial properties and good biocompatibility, functioning through a contact-killing mechanism by disrupting the bacterial membrane.
It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices. Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes. Our recent report on lithium hexamethyldisilazide-initiated superfast polymerization on amino acid N-carboxyanhydrides enables the quick synthesis of host defense peptide-mimicking peptide polymers. Here we reported a facile and cost-effective thermoplastic polyurethane (TPU) surface modification of peptide polymer (DLL: BLG = 90 : 10) using plasma surface activation and substitution reaction between thiol and bromide groups. The peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in vitro. Furthermore, the peptide polymer-modified TPU surfaces showed excellent biocompatibility, displaying no hemolysis and cytotoxicity. In vivo study using methicillin-resistant Staphylococcus aureus (MRSA) for subcutaneous implantation infectious model showed that peptide polymer-modified TPU surfaces revealed obvious suppression of infection and great histocompatibility, compared to bare TPU surfaces. We further explored the antimicrobial mechanism of the peptide polymer-modified TPU surfaces, which revealed a surface contact-killing mechanism by disrupting the bacterial membrane. These results demonstrated great potential of the peptide-modified TPU surfaces for practical application to combat bacterial infections that are associated with implantable materials and devices.
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