期刊
NATURE CATALYSIS
卷 4, 期 10, 页码 872-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41929-021-00685-z
关键词
-
资金
- National Key R&D Program of China [2016YFA0500604]
- National Natural Science Foundation [31420103907, 31770873, 31330022, 31370107]
- Fudan University Original Research Grant [31470777]
- National Natural Science Foundation of China [31870721]
The study presents the in vitro selection of a SAM-dependent methyltransferase ribozyme RNA, which demonstrates site-specific methylation of RNA, showing potential for significant contribution in cellular RNA methylation. The ribozyme shows activity over a wide range of conditions and has the ability to methylate RNA specifically, opening up new possibilities in the field of cellular RNA methylation.
RNA methylations contribute to a wide range of cellular functions. Cellular RNAs are usually methylated by protein methyltransferases using S-adenosyl-l-methionine (SAM) as a cofactor. Here we report the in vitro selection of a 33-nucleotide SAM-dependent methyltransferase ribozyme RNA from a randomized sequence. Detection and mapping of the methyl group on the RNA demonstrates site-specific methylation of the N-7 position of guanine by SAM. The ribozyme is active over a wide range of pH and temperatures and is robust compared to protein enzymes. The ribozyme structures in the presence and absence of SAM show a dramatic local conformational change associated with cofactor binding. The ribozyme motif was found to be distributed in nature, and candidate sequences were shown to be active in vitro. The discovery of this ribozyme that uses the cofactor SAM to specifically methylate RNA opens up the possibility that methyltransferase ribozymes may contribute to cellular RNA methylation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据