期刊
DIVERSITY-BASEL
卷 13, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/d13100501
关键词
amphibian; temperature; SNP; sex determination
资金
- Graduate School, Kasetsart University, Thailand
- Kasetsart University
- Office of the Ministry of Higher Education, Science, Research and Innovation
- Thailand Science Research and Innovation through The Kasetsart University Reinventing University Program 2021
- e-ASIA Joint Research Program (e-ASIA JRP) [P1851311]
The research demonstrates that the sex determination system of the rice field frog is temperature-dependent, with a highly conserved karyotype and lack of sex chromosome heteromorphism. These findings provide valuable insights into the understanding of the mechanism of sex determination in H. rugulosus.
Sex determination systems (SDSs) in anurans are diverse and have undergone independent evolutionary transitions among species. The mode of sexual reproduction of the rice field frog (Hoplobatrachus rugulosus)-an economically viable, edible amphibian species-is not well known. Previous studies have proposed that threshold temperature conditions may determine sex in these frogs. To elucidate the SDS in H. rugulosus, we karyotyped 10 male and 12 female frogs, and performed fluorescence in situ hybridization combined with sequencing analyses using DArTseq (TM). Our results revealed a highly conserved karyotype with no sex chromosome heteromorphism, and the sequencing analyses did not identify any consistent sex-linked loci, supporting the hypothesis of temperature-dependent sex determination. The results of this study, and others, on SDSs in the rice field frog and related species also provide support for the theory that heteromorphic sex chromosomes may lead to an evolutionary trap that prevents variable SDSs. These findings add important information to the body of knowledge on H. rugulosus and are likely to have a significant impact on the productivity and economic success of rice field frog farming.
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