期刊
DIAGNOSTICS
卷 12, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/diagnostics12020239
关键词
exosomes; miRNA; synovial fluid; arthritis
资金
- National Key R&D Program of China [2017YFB0405404]
- Strategic Priority Research Program of the Chinese Academy of Science [XDA16020900, XDB29050301, XDB32030200]
- National Nature Science Foundation of China [31971373, 81803492]
- Yunnan Key Research and Development Program [202003AD150009]
- Innovation Program of Science and Research from the DICP, CAS [DICP I201934]
Arthritis affects approximately 250 million people worldwide and early diagnosis is crucial for treatment. The study found that miRNA expression in synovial fluid differs from that in serum, which can contribute to the research and discovery of early diagnostic biomarkers for joint diseases.
Arthritis is caused by inflammation, infection, degeneration, trauma, or other factors that affect approximately 250 million people all over the world. Early diagnosis and prediction are essential for treatment. Exosomes are nanoscale vesicles that participate in the process of joint disease. Serum is the mainly used sources in the study of arthritis-related exosomes, while whether serum exosomes can reflect the contents of synovial fluid exosomes is still unknown. In this work, we separated exosomes from serum and the synovial fluid of osteoarthritis patients and compared their miRNA expression utilizing miRNA sequencing. The results revealed that 31 upregulated and 33 downregulated miRNAs were found in synovial fluid compared to serum. Transcriptome analysis showed that these differentially expressed miRNAs were mainly associated with intercellular processes and metabolic pathways. Our results show that serum-derived exosomes cannot fully represent the exosomes of synovial fluid, which may be helpful for the study of joint diseases and the discovery of early diagnostic biomarkers of arthritis.
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