Construction of Spirooxindole Analogues Engrafted with Indole and Pyrazole Scaffolds as Acetylcholinesterase Inhibitors

Twenty-five new hits of spirooxindole analogs 8a-y engrafted with indole and pyrazole scaffolds were designed and constructed via a [3+2]-cycloaddition (32CA) reaction starting from three components: new chalconebased indole and pyrazole scaffolds 5a-d, substituted isatins 6a-c, and secondary amines 7a-d. The potency of the compounds were assessed in modulating cholinesterase (AChE) activity using Ellman's method. Compounds 8i and 8y showed the strongest acetylcholine esterase inhibition (AChEI) with IC50 values of 24.1 and 27.8 mu M, respectively. Molecular docking was used to study their interaction with the active site of hAChE.

Automated summary

Twenty-five new hits of spirooxindole analogs engrafted with indole and pyrazole scaffolds were designed and constructed through a [3+2]-cycloaddition reaction. Compounds 8i and 8y showed the strongest acetylcholine esterase inhibition and were further studied with molecular docking for their interaction with the active site of hAChE.