期刊
ACS OMEGA
卷 6, 期 51, 页码 35334-35341出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.1c04335
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资金
- World Institute of Kimchi - Ministry of Science and ICT, Republic of Korea [KE2102-1-1]
- National Research Council of Science & Technology (NST), Republic of Korea [KE2102-1] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study investigated the shelf-life extension effects of a highly efficient and eco-friendly cryoprotective agent and a cold adaptation method on Lactobacillus sakei WiKim31. Results showed that citrus byproduct exhibited high antioxidant activity and could be used as a novel cryoprotective agent either alone or in combination with soy powder to improve the storage conditions of L. sakei WiKim31.
Lactic acid bacteria produce various bioactive compounds widely used in human healthcare. However, studies on cryoprotective agents for the efficient storage of lactic acid bacteria after freeze-drying are still lacking. Here, we report the shelf-life extension effects of a highly efficient and eco-friendly cryoprotective agent and a cold adaptation method on Lactobacillus sakei WiKim31. Cold adaptation of L. sakei WiKim31 increased exopolysaccharide expression in response to abiotic stress. As a possible cryoprotective agent, the citrus byproduct (CP) contains a variety of sugars, amino acids, and cations, exhibiting high antioxidant activity. L. sakei WiKim31 powders formulated with CP or a mixture of soy powder (SP) and CP exhibited high cell viability at 58.3 and 76.3%, respectively, after 56 days of storage. These results indicate that CP can be efficiently used as a novel cryoprotective agent either alone or in combination with SP to improve the storage conditions of L. sakei WiKim31 and preserve it longer.
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