期刊
PLANTS-BASEL
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/plants11030419
关键词
Cannabis sativa; phytochemical fingerprint; growth stage; method of drying; non-psychotomimetic cannabinoids; flavonoids; antioxidant assays; tyrosinase inhibition; UHPLC-UV-ESI-MS; MS
资金
- University of Turin, (Italy)
There is a renewed interest in cannabis-related products due to the rich phytocomplex, fiber, and agricultural features of the plant. The current study chemically characterized fiber-type Cannabis sativa L. samples from Italy and found that the phytocomplex mainly consists of flavonoids and non-psychotomimetic cannabinoids. The freeze-drying method was shown to better preserve the chemical composition of the samples, while the location of the land plot and the growth stage of the plants had minor influences on the chemical pattern. In vitro assays also demonstrated the potential biological activity of these samples and the contribution of non-psychotomimetic cannabinoids.
Currently, there is a renewed interest in cannabis-related products in different fields because of the rich phytocomplex of this plant, together with its fiber and agricultural features. In this context, the current study aims to chemically characterize different samples of fiber-type Cannabis sativa L. grown in Italy as a potential health promoting source. An ultrasound-assisted solid-liquid extraction (UA-SLE) method was first developed and optimized to obtain a fingerprinting of the investigated phytocomplex. Analyses were carried out through an ultra high performance liquid chromatography equipped with a photodiode array detector in series with triple quadrupole system with an electrospray ionization (ESI) interface (UHPLC-UV-ESI-MS/MS) and showed that the phytocomplex mainly includes flavonoids and non-psychotomimetic cannabinoids. The method was then applied to characterize and compare 24 samples of fiber-type Cannabis sativa L. aerial parts (mainly stems and leaves), which differed for the growth stages (from mid-vegetative to early flowering), growth land plots, and methods of drying (forced-draft oven or freeze-drying). The quali-quantitative analysis showed that a freeze-drying method seems to better preserve the chemical composition of the samples, while the location of the land plot and the growth stage of the plant (which did not comprise inflorescences) had minor influences on the chemical pattern. These results were also supported by spectrophotometric in-vitro assays (scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) and 2,2 '-azinobis-3-ethyl-benzthiazoline-6-sulphonate (ABTS(+center dot)) radicals and inhibitory activity against tyrosinase and elastase enzymes) to investigate the potential biological activity of these samples and the contribution of non-psychotomimetic cannabinoids.
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