4.7 Article

Application of Allele Specific PCR in Identifying Offspring Genotypes of Bi-Allelic SbeIIb Mutant Lines in Rice

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Review Biochemistry & Molecular Biology

Current technological interventions and applications of CRISPR/Cas for crop improvement

Priya Shah et al.

Summary: Efficient and innovative genome editing strategies, particularly CRISPR/Cas technology, have emerged as effective tools for improving important traits in plants, potentially revolutionizing agriculture in the face of evolving climatic conditions.

MOLECULAR BIOLOGY REPORTS (2022)

Review Biochemistry & Molecular Biology

The CRISPR-Cas toolbox and gene editing technologies

Guanwen Liu et al.

Summary: The emergence of CRISPR-Cas systems has greatly advanced gene editing technologies in the field of life sciences. Various CRISPR-Cas tools have been engineered to improve their performance, allowing for a broader range of targets, higher efficiency and specificity, and greater precision. Additionally, functional elements have been introduced to enable precise gene modification, gene expression control, and epigenetic changes. This review provides an overview of different types of CRISPR-Cas tools, discusses current limitations, and explores future prospects for optimizing CRISPR-Cas systems.

MOLECULAR CELL (2022)

Review Plant Sciences

Harnessing tissue-specific genome editing in plants through CRISPR/Cas system: current state and future prospects

Dhanawantari L. Singha et al.

Summary: Tissue-specific CRISPR/Cas genome editing is a promising approach for crop improvement, bypassing the issues associated with constitutive genome editing and allowing targeted improvement of crops.

PLANTA (2022)

Article Biotechnology & Applied Microbiology

Development of Kompetitive Allele Specific PCR markers for identification of persimmon varieties using genotyping-by-sequencing

Kyeong Bok Ma et al.

Summary: This study investigated the genetic diversity and variations in persimmon genotypes using SNP markers identified by genotyping-by-sequencing analysis. The developed KASP markers successfully discriminated among persimmon genotypes, providing a reliable identification system for persimmon varieties.

ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2021)

Review Plant Sciences

CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences

Chou Khai Soong Karlson et al.

Summary: RNA-guided genomic transcriptional regulation tools, CRISPRi and CRISPRa, derived from the CRISPR/Cas9 system, utilize a catalytically dead Cas9 to achieve transcriptional interference. Despite its potential benefits, challenges and limitations such as off-target effects, PAM sequence requirements, and labeling as genetically modified organisms are faced by the CRISPR/dCas9 system.

PLANTS-BASEL (2021)

Review Biochemistry & Molecular Biology

A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises

Adnan Rasheed et al.

Summary: Genome editing has provided a novel approach for manipulating the genomes of living organisms, particularly in crop improvement where the CRISPR/Cas9 technique plays a crucial role in enhancing yield, quality, and stress tolerance. By targeting specific genes, this technique can be combined with other breeding methods to boost agricultural productivity and ensure food security, despite facing challenges and limitations that need to be addressed by plant breeders and researchers.

CURRENT ISSUES IN MOLECULAR BIOLOGY (2021)

Article Agronomy

Creating a novel herbicide-tolerance OsALS allele using CRISPR/Cas9-mediated gene editing

Fangquan Wang et al.

Summary: The novel herbicide-tolerance allele (G628W) created in rice using CRISPR/Cas9-mediated gene editing proved to be valuable for developing elite rice varieties with strong herbicide tolerance. A DNA marker for the G628W allele was developed to accurately distinguish homozygous from heterozygous segregants, facilitating its use and introgression into elite alleles for crop breeding. This study further demonstrates the feasibility of CRISPR/Cas9-mediated gene editing in generating novel genetic variation for crop improvement.

CROP JOURNAL (2021)

Review Multidisciplinary Sciences

Review on forward and reverse genetics in plant breeding

Endalkachew Aklilu

Summary: Forward genetics involves identifying the gene responsible for a mutant phenotype, while reverse genetics analyzes the phenotype following disruption of a known gene. Various methods such as chemical treatment and X-rays are used to generate random mutations in organisms, and genetic variation is measured using markers across the genome.

ALL LIFE (2021)

Article Biotechnology & Applied Microbiology

MutMapPlus identified novel mutant alleles of a rice starch branching enzyme IIb gene for fine-tuning of cooked rice texture

Masaru Nakata et al.

PLANT BIOTECHNOLOGY JOURNAL (2018)

Article Biochemistry & Molecular Biology

Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice

Yongyao Xie et al.

JOURNAL OF INTEGRATIVE PLANT BIOLOGY (2017)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Article Agronomy

Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus

K Hayashi et al.

THEORETICAL AND APPLIED GENETICS (2004)

Article Biochemical Research Methods

Efficient validation of single nucleotide polymorphisms in plants by allele-specific PCR, with an example from barley

VD Soleimani et al.

PLANT MOLECULAR BIOLOGY REPORTER (2003)

Article Biochemistry & Molecular Biology

An efficient procedure for genotyping single nucleotide polymorphisms

S Ye et al.

NUCLEIC ACIDS RESEARCH (2001)