4.7 Article

The Novel Cucurbitaceae miRNA ClmiR86 Is Involved in Grafting-Enhanced Phosphate Utilization and Phosphate Starvation Tolerance in Watermelon

期刊

PLANTS-BASEL
卷 10, 期 10, 页码 -

出版社

MDPI
DOI: 10.3390/plants10102133

关键词

phosphate starvation response; phosphate utilization; grafting; watermelon

资金

  1. National Key Research and Development Plan of China [2019YFD1001904, 2019YFD1000303, 2018YFD0201300]
  2. Natural Science Foundation of Zhejiang Province [LY21C150008]
  3. Earmarked Fund for China Agriculture Research System [CARS-25]
  4. Science and Technology Innovation Platform for Watermelon and Melon Breeding, Reproduction and Spreading of Zhejiang Province [2020-KYY-NSFZ-0314]
  5. Key Science and Technology Program for Agricultural (Vegetable) New Variety Breeding of Zhejiang Province [2021C02065]

向作者/读者索取更多资源

Watermelon grafting uses a novel miRNA, ClmiR86, to suppress ClCIPK5 and enhance phosphate-starvation tolerance. The differential expression of ClmiR86 in various watermelon genotypes is correlated with their phosphate utilization efficiency. Overexpression of ClmiR86 in Arabidopsis promotes root growth and phosphate uptake under phosphate starvation.
Watermelon (Citrullus lanatus) is a globally important Cucurbitaceae crop in which grafting is commonly used to improve stress tolerance and enhance nutrient utilization. However, the mechanism underlying grafting-enhanced nutrient assimilation remains unclear. Here, we demonstrate the possible involvement of a novel Cucurbitaceae miRNA, ClmiR86, in grafting-enhanced phosphate-starvation tolerance via CALCINEURIN B-LIKE INTERACTING PROTEIN KINASE 5 (ClCIPK5) suppression in watermelon. Transcript analyses revealed that the induction of ClmiR86 expression was correlated with the downregulation of ClCIPK5 in squash-grafted watermelon under phosphate starvation. In addition, the differential expression of ClmiR86 in various watermelon genotypes was consistent with their phosphate utilization efficiency. Furthermore, ClmiR86 overexpression in Arabidopsis enhanced root growth and phosphate uptake under phosphate starvation and promoted inflorescence elongation under normal conditions. These results suggest that the ClmiR86-ClCIPK5 axis is involved in phosphate starvation response as well as grafting-enhanced growth vigor and phosphate assimilation. The present study provides valuable insights for investigating long-distance signaling and nutrient utilization in plants.

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