Contribution of Ascorbate and Glutathione in Endobacteria Bacillus subtilis-Mediated Drought Tolerance in Two Triticum aestivum L. Genotypes Contrasting in Drought Sensitivity

We evaluated the effect of endobacteria Bacillus subtilis (strain 10-4) as a co-inoculant for promoting plant growth and redox metabolism in two contrasting genotypes of Triticum aestivum L. (wheat): Ekada70 (drought tolerant (DT)) and Salavat Yulaev (drought susceptible (DS)) in early stages of adaptation to drought (12% PEG-6000). Results revealed that drought reduced growth and dramatically augmented oxidative stress markers, i.e., hydrogen peroxide (H2O2) and lipid peroxidation (MDA). Furthermore, the depletion of ascorbate (AsA) and glutathione (GSH), accompanied by a significant activation of ascorbate peroxidase (APX) and glutathione reductase (GR), in both stressed wheat cultivars (which was more pronounced in DS genotype) was found. B. subtilis had a protective effect on growth and antioxidant status, wherein the stabilization of AsA and GSH levels was revealed. This was accompanied by a decrease of drought-caused APX and GR activation in DS plants, while in DT plants additional antioxidant accumulation and GR activation were observed. H2O2 and MDA were considerably reduced in both drought-stressed wheat genotypes because of the application of B. subtilis. Thus, the findings suggest the key roles in B. subtilis-mediated drought tolerance in DS cv. Salavat Yulaev and DT cv. Ekada70 played are AsA and GSH, respectively; which, in both cases, resulted in reduced cell oxidative damage and improved growth in seedlings under drought.

Automated summary

Bacillus subtilis co-inoculant alleviates oxidative stress and promotes growth in wheat under drought conditions, with key roles played by ascorbate and glutathione in enhancing drought tolerance.