期刊
PLANTS-BASEL
卷 10, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/plants10112532
关键词
Ficus carica L. waste leaves; antioxidant; HPLC-QTOF-MS/MS-DPPH; flavonoids; polyphenols
资金
- Fundamental Research Fund for Central Universities [2572019CZ01]
- Heilongjiang Touyan Innovation Team Program [B20088]
The study evaluated the antioxidant capacity of different extracts from fig waste leaves, revealing that ethyl acetate extraction contained the highest content of flavonoids. A rapid and efficient method was developed to identify antioxidant components in fig waste leaves, demonstrating the presence of various flavonoids with significant antioxidant capacity.
Fig (Ficus carica L.) leaves are produced each year and often disposed, resulting in a waste of resources. Fig waste leaves are rich in flavonoids, which have strong antioxidant activity; however, the variety and chemical structure of antioxidants in fig leaves have not been reported in detail. To take full advantage of fig waste leaves, antioxidant capacity of different extracts (petroleum ether, ethyl acetate, and water) was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2 & PRIME;-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), and ferric-ion-reducing antioxidant power (FRAP) methods. The results showed that flavonoids in ethyl acetate extraction had the highest content (83.92 & PLUSMN; 0.01 mg/g), maximum DPPH scavenging activity (IC50 0.54 mg/mL), highest ABTS scavenging rate (80.28%), and FRAP (3.46 mmol/g). Furthermore, an HPLC-QTOF-MS/MS-DPPH method was developed to identify 11 flavonoids in fig waste leaves. This rapid and efficient method can not only be used for screening the antioxidant components in fig waste leaves, but also can be combined with mass spectrometry to identify the compounds with antioxidant capacity. There are three flavonoids with significant antioxidant capacity, which are 3-O-(rhamnopyranosyl-glucopyranosyl)-7-O-(glucopyranosyl)-quercetin, isoschaftoside, and rutin. The results confirmed that fig waste leaves contain a variety of antioxidant components, which contributed to increase the value of fig waste leaves as antioxidants.
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