Preparation and antibacterial application of hydroxyapatite doped Silver nanoparticles derived from chicken bone

Hydroxyapatite was isolated from the chicken bones of Black Sumatra and Fighter cock by thermal cal-cinations at 700 degrees C. The reducing power of Plumbago indica was responsible for the green synthesis of sil-ver NPs. The isolated hydroxyapatite was used for the preparation of hydroxyapatite doped silver nanoparticles (HAp/AgNPs) against both Gram-positive and Gram-negative bacteria. The Black Sumatra bone derived HAp/AgNPs were excellent activity against Klebsiella pneumoniae (28 mm), Staphylococcus aureus (26 mm), Bacillus cereus (24 mm), respectively. Fighting cock bones derived HAp/AgNPs demon-strated activity against Staphylococcus aureus (22 mm), Klebsiella pneumoniae (25 mm) and Bacillus cereus (23 mm), respectively. The MIC value ranged between 45 +/- 1.2 mg/mL and 104 +/- 0.25 mg/mL. The HAp/ AgNPs were characterized by X-ray diffraction (XRD), Fourier Transformed Infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). The XRD patterns of the Hap/Ag revealed functional peaks representing the reflection planes. The HAp/AgNPs showed an ellipsoidal morphology in SEM anal-ysis. The morphological studies at various magnifications indicated that HAp/AgNPs with good crystal structure could prepared by using co-precipitation method. (c) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

This study isolated hydroxyapatite from the bones of Black Sumatra and Fighter cock and synthesized silver nanoparticles using the reducing power of Plumbago indica. The hydroxyapatite doped silver nanoparticles showed antibacterial activity against both Gram-positive and Gram-negative bacteria.