期刊
ANTIBIOTICS-BASEL
卷 11, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/antibiotics11020228
关键词
bacteriophages; pathogenic bacteria; phage resistance; Campylobacter; food safety; phage cocktail; phage selection; cocktail design; firehammervirus; fletchervirus
资金
- German Federal Ministry of Education and Research (BMBF) [IP5/01KI1725C]
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [LE 824/10-1]
- University of Veterinary Medicine Hannover, Foundation
This study aimed to investigate the effects of newly isolated phages on current Campylobacter strains and compare the accuracy of different assay methods. The results showed that planktonic killing assays were a simple and effective tool for phage cocktail design. The mixture of group II phage vB_CcM-LmqsCP218-2c2 and group III phage vB_CjM-LmqsCP1-1 showed the most promise for combating Campylobacter infections.
Campylobacteriosis is a worldwide-occurring disease and has been the most commonly reported zoonotic gastrointestinal infection in the European Union in recent years. The development of successful phage-based intervention strategies will require a better understanding of phage-bacteria interactions to facilitate advances in phage cocktail design. Therefore, this study aimed to investigate the effects of newly isolated group II and group III phages and their combinations on current Campylobacter field strains. A continuous workflow for host range and efficiency of plating (EOP) value determination was combined with a qPCR-based phage group identification and a liquid-based planktonic killing assay (PKA). An advanced analysis scheme allowed us to evaluate phage cocktails by their efficacy in inhibiting bacterial population growth and the resulting phage concentrations. The results of this study indicate that data obtained from PKAs are more accurate than host range data based on plaque formation (EOP). Planktonic killing assays with Campylobacter appear to be a useful tool for a straightforward cocktail design. Results show that a group II phage vB_CcM-LmqsCP218-2c2 and group III phage vB_CjM-LmqsCP1-1 mixture would be most promising for practical applications against Campylobacter coli and Campylobacter jejuni.
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