期刊
ANTIBIOTICS-BASEL
卷 11, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/antibiotics11020245
关键词
Xanthomonas oryzae; antibiotic resistance; Zhongshengmycin; P cycle; alanine
资金
- National Natural Science Foundation of China [32072481]
Microbial antibiotic resistance has become a worldwide concern. This study reveals that the decline in the pyruvate cycle and the decrease in alanine level are important mechanisms for Zhongshengmycin resistance in Xanthomonas oryzae.
Microbial antibiotic resistance has become a worldwide concern, as it weakens the efficiency of the control of pathogenic microbes in both the fields of medicine and plant protection. A better understanding of antibiotic resistance mechanisms is helpful for the development of efficient approaches to settle this issue. In the present study, GC-MS-based metabolomic analysis was applied to explore the mechanisms of Zhongshengmycin (ZSM) resistance in Xanthomonas oryzae (Xoo), a bacterium that causes serious disease in rice. Our results show that the decline in the pyruvate cycle (the P cycle) was a feature for ZSM resistance in the metabolome of ZSM-resistant strain (Xoo-ZSM), which was further demonstrated as the expression level of genes involved in the P cycle and two enzyme activities were reduced. On the other hand, alanine was considered a crucial metabolite as it was significantly decreased in Xoo-ZSM. Exogenous alanine promoted the P cycle and enhanced the ZSM-mediated killing efficiency in Xoo-ZSM. Our study highlights that the depressed P cycle is a feature in Xoo-ZSM for the first time. Additionally, exogenous alanine is a candidate enhancer and can be applied with ZSM to improve the antibiotic-mediated killing efficiency in the control of infection caused by Xoo.
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