期刊
FOODS
卷 10, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/foods10123155
关键词
Poriae Cutis; triterpenoid; purification; identification; anti-inflammatory
资金
- National Key Research and Development Plan of China [2017YFC1703006]
- Agricultural Science and Technology Innovation Center of Hubei Province [2016-620-000-001-044]
- Hubei Agriculture Research System [HBHZDZB-2021-023]
Triterpenoid compounds from Poriae Cutis were separated and identified, with poricoic acid B showing higher anti-inflammatory activity compared to poricoic acid A, while dehydrotrametenolic acid and dehydroeburicoic acid had no anti-inflammatory properties. In addition, in vitro experiments demonstrated that poricoic acid B could decrease the production of cytokines in a dose-dependent manner.
In this study, triterpenoid compounds from Poriae Cutis were separated by high-speed countercurrent chromatography (HSCCC) and identified using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) and nuclear magnetic resonance (NMR). The in vitro anti-inflammatory activities of the purified triterpenoids on RAW 264.7 cells were also investigated. Triterpenoids, poricoic acid B, poricoic acid A, dehydrotrametenolic acid, and dehydroeburicoic acid were obtained; their levels of purity were 90%, 92%, 93%, and 96%, respectively. The results indicated that poricoic acid B had higher anti-inflammatory activity than those of poricoic acid A by inhibiting the generation of NO in lipopolysaccharide (LPS)-induced RAW 264.7 cells. However, dehydrotrametenolic acid and dehydroeburicoic acid had no anti-inflammatory activity. In addition, the production of cytokines (TNF-alpha, IL-1 beta, and IL-6) in cells treated with poricoic acid B decreased in a dose-dependent manner in the concentration range from 10 to 40 mu g/mL. The results provide evidence for the use of Poriae Cutis as a natural anti-inflammatory agent in medicines and functional foods.
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