期刊
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
卷 26, 期 7, 页码 1190-1195出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/IGC.0000000000000774
关键词
YKL-40; siRNA; Endometrial cancer; Proliferation; Migration; Invasion
资金
- Natural Scientific Foundation of China [81360388]
Objective The purpose of this study was to explore the effects of a small interfering RNA (siRNA) targeting YKL-40 on the proliferation and invasion of endometrial cancer (EC) HEC-1A cells. Methods We used an siRNA targeting a sequence in YKL-40 (si-YKL-40) to transfect HEC-1A cells. Quantitative real-time polymerase chain reaction assay was performed to investigate the mRNA levels of YKL-40. MTT, migration, and invasion assays were performed to identify the effects of si-YKL-40 on the proliferation, migration, and invasive abilities of the HEC-1A cells. Results mRNA expression of YKL-40 was down-regulated in HEC-1A cells after transfection with si-YKL-40 (P < 0.05). The proliferation, migration, and invasive abilities of HEC-1A cells were inhibited by siRNA (P < 0.05). ConclusionsYKL-40 targeting siRNA specifically blocks the activity of YKL-40 in human EC HEC-1A cells, resulting in tumor suppression. This indicates that YKL-40 might serve as a potential small molecule target in the treatment of EC.
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