期刊
METABOLITES
卷 11, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/metabo11100707
关键词
Microchloropsis gaditana; Brachionus plicatilis; volatile organic compounds; SPME-GCMS
资金
- Sandia Laboratories Lab Directed Research and Development (LDRD) project [199974]
- Lawrence Livermore National Laboratory LDRD project [19-FS-035]
- US Department of Energy's Genomic Science Program [SCW1039]
- Michigan AgBioResearch through the USDA National Institute of Food and Agriculture, Hatch project [MICL02474]
Open microalgal ponds are vulnerable to various biotic and abiotic stressors, leading to crashes with high economic costs. This study identified specific VOCs for early diagnosis of algal crop damage, demonstrating unique diagnostic VOCs for different damage mechanisms. Detecting these VOCs allows for targeted treatment, ultimately preventing pond crashes and enhancing algal crop yields for biofuel production.
Open microalgal ponds used in industrial biomass production are susceptible to a number of biotic and abiotic environmental stressors (e.g., grazers, pathogens, pH, temperature, etc.) resulting in pond crashes with high economic costs. Identification of signature chemicals to aid in rapid, non-invasive, and accurate identification of the stressors would facilitate targeted and effective treatment to save the algal crop from a catastrophic crash. Specifically, we were interested in identifying volatile organic compounds (VOCs) that can be used to as an early diagnostic for algal crop damage. Cultures of Microchloropsis gaditana were subjected to two forms of algal crop damage: (1) active grazing by the marine rotifer, Brachionus plicatilis, or (2) repeated freeze-thaw cycles. VOCs emitted above the headspace of these algal cultures were collected using fieldable solid phase microextraction (SPME) fibers. An untargeted analysis and identification of VOCs was conducted using gas chromatography-mass spectrometry (GC-MS). Diagnostic VOCs unique to each algal crop damage mechanism were identified. Active rotifer grazing of M. gaditana was characterized by the appearance of carotenoid degradation products, including beta-cyclocitral and various alkenes. Freeze-thaw algae produced a different set of VOCs, including palmitoleic acid. Both rotifer grazing and freeze-thawed algae produced beta-ionone as a VOC, possibly suggesting a common stress-induced cellular mechanism. Importantly, these identified VOCs were all absent from healthy algal cultures of M. gaditana. Early detection of biotic or abiotic environmental stressors will facilitate early diagnosis and application of targeted treatments to prevent algal pond crashes. Thus, our work further supports the use of VOCs for monitoring the health of algal ponds to ultimately enhance algal crop yields for production of biofuel.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据