4.5 Article

Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Infectious Diseases

LAMP assay coupled with CRISPR/Cas12a system for portable detection of African swine fever virus

Bo Yang et al.

Summary: A method for the convenient, portable, low cost, highly sensitive and specific detection of African swine fever virus (ASFV) was established using a combined LAMP-CRISPR assay. The detection limit reached 7 copies/microliter of p72 gene per reaction with no cross-reactivity with other porcine viruses. The performance of this method showed good consistency with real-time qPCR tests, demonstrating a great potential for on-site monitoring of ASFV in the field.

TRANSBOUNDARY AND EMERGING DISEASES (2022)

Article Infectious Diseases

Lateral flow assays for the detection of African swine fever virus antigen are not fit for field diagnosis of wild boar carcasses

Paul Deutschmann et al.

Summary: African swine fever (ASF) is an important viral disease for domestic pigs and wild boar, emphasizing the need for early pathogen detection. The 'point-of-care' tests are discussed but currently cannot substitute for OIE-listed diagnostic methods, with limited use for carcass testing.

TRANSBOUNDARY AND EMERGING DISEASES (2022)

Article Infectious Diseases

Rapid and highly sensitive portable detection of African swine fever virus

Jade Daigle et al.

Summary: African swine fever (ASF) is spreading in Asia, devastating pig populations with no effective vaccine available. Early detection is crucial, and a portable pen-side thermocycler has been successfully used for rapid ASF detection in clinical samples. The field-deployable molecular assay can improve response time by eliminating the need to ship samples to a central laboratory.

TRANSBOUNDARY AND EMERGING DISEASES (2021)

Article Microbiology

First-Time Presence of African Swine Fever Virus Genotype II in Nigeria

Adeyinka J. Adedeji et al.

Summary: The discovery of African swine fever virus genotype II in Nigeria has serious implications for control measures and food security in the region.

MICROBIOLOGY RESOURCE ANNOUNCEMENTS (2021)

Article Infectious Diseases

Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam

Diem Hong Tran et al.

Summary: African swine fever (ASF) is a highly infectious viral disease with a recent outbreak in Vietnam. A modified colorimetric loop-mediated isothermal amplification (LAMP) assay has shown potential for rapid field diagnosis of ASF, with results visible to the naked eye within 30 minutes, without the need for complex equipment or DNA extraction.

TRANSBOUNDARY AND EMERGING DISEASES (2021)

Article Virology

Development of A Super-Sensitive Diagnostic Method for African Swine Fever Using CRISPR Techniques

Meishen Ren et al.

Summary: A nucleic acid detection method using RPA-CRISPR was developed for diagnosing African swine fever, demonstrating high sensitivity and the ability to distinguish ASFV genomic DNA from other porcine viral DNA/RNA without cross-reactivity. The method showed 100% coincidence rate for diagnosing ASFV clinical DNA samples, highlighting its potential for clinical quarantine of ASFV in the swine industry and food security.

VIROLOGICA SINICA (2021)

Article Immunology

An Isothermal Molecular Point of Care Testing for African Swine Fever Virus Using Recombinase-Aided Amplification and Lateral Flow Assay Without the Need to Extract Nucleic Acids in Blood

Yuhang Zhang et al.

Summary: In this study, a sensitive, specific, rapid, and simple molecular point of care testing for African swine fever virus (ASFV) B646L gene in blood samples was established without the need to extract viral DNA, providing a good choice for ASF diagnosis in the future. The method showed 100% coincident rate with OIE-recommended PCR, had higher sensitivity, and exhibited good specificity in clinical blood samples evaluation.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

Article Chemistry, Analytical

Portable Magnetofluidic Device for Point-of-Need Detection of African Swine Fever

Liben Chen et al.

Summary: African swine fever (ASF), with a nearly 100% mortality rate, has devastated pork industries around the world. In the absence of a vaccine, a portable device has been developed for rapid ASF detection, exhibiting high diagnostic accuracy in farm and slaughter facility settings.

ANALYTICAL CHEMISTRY (2021)

Article Multidisciplinary Sciences

Rapid and sensitive RPA-Cas12a-fluorescence assay for point-of-care detection of African swine fever virus

Jinyu Fu et al.

Summary: The RPA-Cas12a-fluorescence assay based on the p72 gene sequence provides a fast and visible tool for ASFV detection, showing high sensitivity and specificity for on-site rapid testing under isothermal conditions.

PLOS ONE (2021)

Article Infectious Diseases

Evaluation of oral fluid as an aggregate sample for early detection of African swine fever virus using four independent pen-based experimental studies

Kalhari B. Goonewardene et al.

Summary: The study showed that pen-based oral fluid samples performed well in early detection of ASFV in animal experiments, suggesting they can be used to supplement traditional samples for non-invasive screening.

TRANSBOUNDARY AND EMERGING DISEASES (2021)

Article Virology

Meat Exudate for Detection of African Swine Fever Virus Genomic Material and Anti-ASFV Antibodies

Chukwunonso Onyilagha et al.

Summary: Research has shown that meat exudate can be used as an alternative sample type for detecting ASFV-specific nucleic acids and antibodies when routine sample types are unavailable. Pigs infected with different ASFV strains exhibited varying clinical signs and survival outcomes.

VIRUSES-BASEL (2021)

Article Immunology

Replication and virulence in pigs of the first African swine fever virus isolated in China

Dongming Zhao et al.

EMERGING MICROBES & INFECTIONS (2019)

Article Agriculture, Dairy & Animal Science

African Swine Fever Epidemiology and Control

Linda K. Dixon et al.

Annual Review of Animal Biosciences (2019)

Article Infectious Diseases

Why is African swine fever still present in Sardinia?

C. Jurado et al.

TRANSBOUNDARY AND EMERGING DISEASES (2018)

Editorial Material Infectious Diseases

African swine fever: an unprecedented disaster and challenge to China

Tao Wang et al.

INFECTIOUS DISEASES OF POVERTY (2018)

Article Veterinary Sciences

Development of a novel lateral flow assay for detection of African swine fever in blood

P. Sastre et al.

BMC VETERINARY RESEARCH (2016)

Article Microbiology

Control of African swine fever epidemics in industrialized swine populations

Tariq Halasa et al.

VETERINARY MICROBIOLOGY (2016)

Article Biochemical Research Methods

A quantitative lateral flow assay to detect complement activation in blood

Elizabeth C. Schramm et al.

ANALYTICAL BIOCHEMISTRY (2015)

Review Pathology

An Update on the Epidemiology and Pathology of African Swine Fever

J. M. Sanchez-Vizcaino et al.

JOURNAL OF COMPARATIVE PATHOLOGY (2015)

Article Food Science & Technology

Scientific Opinion on African swine fever

Edith Authie et al.

EFSA JOURNAL (2014)

News Item Multidisciplinary Sciences

Pig fever sweeps across Russia

Ewen Callaway

NATURE (2012)

Article Veterinary Sciences

Review of African swine fever : transmission, spread and control : review article

M-L. Penrith et al.

Journal of the South African Veterinary Association (2012)

Article Food Science & Technology

Scientific Opinion on African Swine Fever

Philippe Vannier et al.

EFSA JOURNAL (2010)

Article Biochemical Research Methods

Quantitative simultaneous multiplex real-time PCR for the detection of porcine cytokines

JC Duvigneau et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2005)

Article Entomology

Ornithodoros porcinus ticks, bushpigs, and African swine fever in Madagascar

F Roger et al.

EXPERIMENTAL AND APPLIED ACAROLOGY (2001)